Research Article
IGF1 and NRG1 Enhance Proliferation, Metabolic Maturity, and the Force-Frequency Response in hESC-Derived Engineered Cardiac Tissues
Figure 5
IGF1 stimulates cardiomyocyte proliferation in engineered tissues. Immunohistological staining of cardiac troponin T (pink), nuclei (blue), and bromodeoxyuridine- (BrdU-) positive nuclei (brown) in control (a) and IGF1-treated (b) ECTs. (c) Percent BrdU-positive nuclei was calculated for each group by counting the number of BrdU-positive cardiac nuclei of the total cardiac nuclei ( biological replicates with ≥100 cardiomyocytes counted per group). .
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