Research Article
Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+/ABCB5+ Limbal Epithelial Stem Cell Cultures
Figure 2
Marker analysis of ABCG2+/ABCB5+ LESCs. (a, c) Total mRNA was isolated from ABCG2+/ABCB5+ LESCs cultured in 10% DMEM, CnT20, or CnT30, and gene expression was assessed by RT-PCR. versus DMEM (b) ABCG2+/ABCB5+ LESCs were immunofluorescently stained with antibodies to p63α, ABCG2, and CK19. Scale bar = 50 μm. w/o Ab: without primary antibody + secondary antibody.
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