Research Article

WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells

Figure 3

WDR62 knockdown effects hPSC specification to neural and glial progenitors. (a) Q-PCR data suggest significant reduction of TBR2 (), S100β (), and CASP3 () expression in Dox-treated WDR62 shRNA-transduced hPSC-derived neural progenitors treated with Dox versus no treatment at day 14 neural induction (N ≥ 3 independent experiments). Two-tailed t-test was used to assess significance. (b) Similarly, Q-PCR data suggest significant reduction of TBR2 () and S100β () expression in Dox-treated versus no Dox treatment at the NSP stage (N ≥ 3 independent experiments, >3 NSPs per experiment). Two-tailed t-test was used to assess significance. ; . NI: neural induction; NSPs: neurospheres; Dox: doxycycline.
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