Maternal Adaptive Immune Cells in Decidua Parietalis Display a More Activated and Coinhibitory Phenotype Compared to Decidua Basalis

Figure 2

Lymphocytes in decidua parietalis express more coinhibitory markers compared to basalis. (a) OPLS plot showing associations between decidual compartment and phenotypic coinhibitory markers (n = 10–12). (b) Surface expression and representative histograms and contour plots showing the expression of the indicated extracellular markers on decidua basalis and parietalis T cells (CD3⁺) compared to the fluorescent minus one (FMO) control of PD-1 (), LAG-3 (), TIM-3 (), and PD-1⁺TIM-3⁺ (). (c) B cell (CD19⁺) surface expression of PD-1 (), TIM-3 (), CTLA-4 (), and PD-1⁺TIM-3⁺ (). (d) NK cell (CD56⁺CD3⁻) surface expression of LAG-3 (). (e) Representative histograms and contour plots showing the expression of the indicated extracellular markers on decidua basalis and parietalis cells from (c) to (d) compared to the fluorescent minus one (FMO) control. Line in graphs depicts the median. Comparisons between the paired samples were made using the nonparametric Wilcoxon test. ns = not significant; ; ; .

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