Angiogenic Capacity of Dental Pulp Stem Cell Regulated by SDF-1α-CXCR4 Axis

Figure 1

Primary isolation and characterization of DPSCs. Primary isolated DPSCs were cultured and characterized. (a) DPSCs showed typical MSC-like morphology at passage 3. (b) The expression of surface antigens was determined by FACS analysis. DPSCs were positive for MSC markers (CD29, CD44, CD73, CD90, and CD105) but negative for hematopoietic cell markers (CD14, CD34, CD45, CD117, and HLA-DR) and endothelial cell marker (CD31). DPSCs were induced in osteogenic or adipogenic medium for 21 days. (c) Alizarin red staining revealed deposits of calcium. (d) Oil red O staining showed lipid vacuoles. (e) Alcian blue staining confirmed chondrogenic differentiation.

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