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Stem Cells International
Volume 2017, Article ID 8154569, 8 pages
Research Article

Therapeutic Potential of Multilineage-Differentiating Stress-Enduring Cells for Osteochondral Repair in a Rat Model

1Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
2Department of Surgery, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
3Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan
4Department of Stem Cell Biology and Histology, Graduate School of Medicine, Tohoku University, Sendai, Japan

Correspondence should be addressed to Naosuke Kamei;

Received 26 January 2017; Revised 27 September 2017; Accepted 28 September 2017; Published 29 October 2017

Academic Editor: Heinrich Sauer

Copyright © 2017 Elhussein Elbadry Mahmoud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Multilineage-differentiating stress-enduring (Muse) cells are stage-specific embryonic antigen-3 (SSEA-3) positive cells existing in mesenchymal stem cell (MSC) populations. Muse cells have the pluripotency to differentiate into all germ layers as embryonic stem cells. In this study, we aimed to investigate the efficacy of Muse cell transplantation for osteochondral defect repair. Muse cells were isolated from human bone marrow MSCs. An osteochondral defect was created in the patellar groove of immunodeficient rats. After this, cell injection was performed, whereby rats were divided into 3 groups: the control group, the rats of which were given a PBS injection; the non-Muse group, which comprised 5 × 104 SSEA-3 negative non-Muse cells; and the Muse group, which comprised 5 × 104 SSEA-3 positive Muse cells. The white repaired tissue had a mostly smooth homogenous surface at 12 weeks after treatment in the Muse group, while no repair tissue was detected in the control and non-Muse groups. Histological assessments showed better repair at the cartilage defect sites in the Muse group compared to the other groups at 4 and 12 weeks after treatment. Muse cells could be a new promising cell source for the treatment of osteochondral defects.