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Stem Cells International
Volume 2017, Article ID 8596135, 11 pages
Research Article

The DEAD-Box RNA Helicase DDX3 Interacts with m6A RNA Demethylase ALKBH5

1CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China
2Anhui Province Key Laboratory of Brain Function and Brain Disease, Hefei, Anhui 230001, China
3Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui 230001, China

Correspondence should be addressed to Liang Chen; nc.ude.ctsu@lcgniqna and Ge Shan; nc.ude.ctsu@egnahs

Received 16 May 2017; Revised 27 July 2017; Accepted 17 August 2017; Published 23 November 2017

Academic Editor: Yujing Li

Copyright © 2017 Abdullah Shah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


DDX3 is a member of the family of DEAD-box RNA helicases. DDX3 is a multifaceted helicase and plays essential roles in key biological processes such as cell cycle, stress response, apoptosis, and RNA metabolism. In this study, we found that DDX3 interacted with ALKBH5, an m6A RNA demethylase. The ATP domain of DDX3 and DSBH domain of ALKBH5 were indispensable to their interaction with each other. Furthermore, DDX3 could modulate the demethylation of mRNAs. We also showed that DDX3 regulated the methylation status of microRNAs and there was an interaction between DDX3 and AGO2. The dynamics of m6A RNA modification is still a field demanding further investigation, and here, we add a link by showing that RNA demethylation can be regulated by proteins such as DDX3.