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Stem Cells International
Volume 2017, Article ID 8740294, 9 pages
Research Article

Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair

1Department of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
2Medical Center for Translational & Clinical Research, Hiroshima University Hospital, Hiroshima, Japan
3Japan Tissue Engineering Co., Ltd., Gamagori, Japan
4Uonuma Institute of Community Medicine, Niigata University Medical and Dental Hospital, Niigata, Japan
5Department of Health and Sports Sciences, Mukogawa Women’s University, Nishinomiya, Japan

Correspondence should be addressed to Naosuke Kamei;

Received 10 May 2017; Accepted 12 June 2017; Published 1 August 2017

Academic Editor: Celeste Scotti

Copyright © 2017 Katsunori Shiraishi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of MYBL1 was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of RCAN2 and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of HLA-DRB1 was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of TMEM155 and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair.