Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9

Table 1

Candidates and oligos for MYOGENIN exon1 target positions for CRISPR/Cas9.

Position		Target sequence	Sequence information		Number of target sites
Start–end	+/−	20 bp + PAM	GC (%)	Tm (°C)	20 bp	12 bp	8 bp
143–165	−	cctgcctgtccacctccagggct	70	84	1	61	5171
147–169	−	cctgtccacctccagggcttcga	65	80	1	38	92,481
152–174	−	ccacctccagggcttcgaaccac	65	79	1	89	10,513
155–177	−	cctccagggcttcgaaccaccag	65	79	1	8	11,331
156–178	+	ctccagggcttcgaaccaccagg	65	79	1	1	4654
158–180	−	ccagggcttcgaaccaccaggct	65	81	1	7	21,861
166–188	+	tcgaaccaccaggctacgagcgg	60	77	1	10	12,349
170–192	+	accaccaggctacgagcggacgg	65	82	1	1	3812
171–193	−	ccaccaggctacgagcggacgga	70	82	1	8	1537
174–196	−	ccaggctacgagcggacggagct	70	83	1	3	479
191–213	+	ggagctcaccctgagccccgagg	75	84	1	43	382

pX458-hMYOG+189_F primer: CACCaccaccaggctacgagcgga. pX458-hMYOG+189_R primer: AAACtccgctcgtagcctggtggt.