Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype

Table 1

Primers for qRT-PCR.

Gene	Forward (5→ 3)	Reverse (5→ 3)
Cytokines
Adiponectin	AACATGCCCATTCGCTTTAC	AGAGGCTGACCTTCACATCC
Chitinase 3-like 1	TGTGAAGGCGTCTCAAACAG	TACAGAGGAAGCGGTCAAGG
Complement factor D	GGTCACCCAAGCAACAAAGT	CCATGCTGATCTCGAACTCC
IGFBP-3	TAAGTATGGGCAGCCTCTCC	GGTCATGTCCTTGGCAGTCT
IL-6	GTAGCCGCCCCACACAGACAGCC	GCCATCTTTGGAAGGTTC
Leptin	GAAGACCACATCCACACACG	AGCTCAGCCAGACCCATCTA
IL-24	GCCTCTGATTGGTGAATGGT	GGTGTTAAATTGGCGAAAGC
Pentraxin-3	ATTCAGAGGAAGGGCTCACA	TGTTTCATCAAAGCCACCAC
CCL5	CGCTGTCATCCTCATTGCTA	GCACTTGCCACTGGTGTAGA
SerpinE1	CAGACCAAGAGCCTCTCCAC	GACTGTTCCTGTGGGGTTGT
CCL17	CACCCCAGACTCCTGACTGT	CATGGCTCCAGTTCAGACAA
CAF markers
PDGFRβ	GCACTTTTATCCACCCAGGA	GTACTTGGCTCAGCCTCCAG
NG2	AGTATGGGCATCTCCTGGTG	CATTGACACCCCTAGCCAGT
α-SMA	AATGGCTCTGGGCTCTGTAA	TTTGCTCTGTGCTTCGTCAC
VEGF	CGAGGGCCTGGAGTGTGT	CCGCATAATCTGCATGGTGAT
FAP	TACCCAAAGGCTGGAGCTAA	ACAGGACCGAAACATTCTGG
FSP	CAAGTACTCGGGCAAAGAGG	TGCAGGACAGGAAGACACAG
Housekeeping gene
β-actin	CACCTTCTACAATGAGCTGC	TCTTCTCGATGCTCGACGGA

CAF = carcinoma-associated fibroblast.