Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells
Phase contrast images of corneal stroma cell cultures and respective growth rates. Pictures show days 1, 8, 15, and 21 of cultivation for cells obtained from the central and peripheral regions of the stroma by enzymatic digestion and explant techniques (a). The scale bars represent 1000 μm. The explanted tissue is marked by an asterisk (∗). Confluency of the cultured cells % ± SD was determined by ImageJ measurements for each isolation technique (), and the points were plotted accordingly for the given values on the respective days (b).