Research Article

Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells

Figure 3

Immunofluorescent staining of nuclear Ki-67 in cultured cells. CSCs obtained from either the central or peripheral corneal stroma by digestion and explant techniques have been cultured for 21–30 days, respectively, and stained for proliferation marker Ki-67. The proliferation marker is shown in red with DAPI counterstaining (a). Relative quantity of Ki-67-positive cells (%) ± SD is shown (b) (). The scale bars represent 200 μm.