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Stem Cells International
Volume 2017 (2017), Article ID 9312329, 13 pages
https://doi.org/10.1155/2017/9312329
Research Article

Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

1Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan
2Department of Orthopaedic Surgery, Wakamatsu Hospital, University of Occupational and Environmental Health, Kitakyushu, Japan
3Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, MO, USA
4Department of Cartilage Regeneration, Tokyo Medical and Dental University, Tokyo, Japan

Correspondence should be addressed to Soshi Uchida

Received 17 July 2016; Revised 26 October 2016; Accepted 29 November 2016; Published 1 January 2017

Academic Editor: Shiwu Dong

Copyright © 2017 Akihisa Hatakeyama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To determine the characteristics of MSCs from hip and compare them to MSCs from knee. Methods. Synovial tissues were obtained from both the knee and the hip joints in 8 patients who underwent both hip and knee arthroscopies on the same day. MSCs were isolated from the knee and hip synovial samples. The capacities of MSCs were compared between both groups. Results. The number of cells per unit weight at passage 0 of synovium from the knee was significantly higher than that from the hip (). While it was possible to observe the growth of colonies in all the knee synovial fluid samples, it was impossible to culture cells from any of the hip samples. In adipogenesis experiments, the frequency of Oil Red-O-positive colonies and the gene expression of adipsin were significantly higher in knee than in hip. In osteogenesis experiments, the expression of COL1A1 and ALPP was significantly less in the knee synovium than in the hip synovium. Conclusions. MSCs obtained from hip joint have self-renewal and multilineage differentiation potentials. However, in matched donors, adipogenesis and osteogenesis potentials of MSCs from the knees are superior to those from the hips. Knee synovium may be a better source of MSC for potential use in hip diseases.