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Stem Cells International
Volume 2017, Article ID 9717353, 9 pages
https://doi.org/10.1155/2017/9717353
Research Article

Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1

1Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA), Cardiovascular Sciences, University of Liège (ULg), Liège, Belgium
2Division of Nephrology, University of Liège Hospital (ULg CHU), Liège, Belgium
3Centre de Recherche en Cancérologie de Marseille, Aix Marseille Université UM105, Institut Paoli Calmettes, UMR7258 CNRS, U1068 INSERM, Cell Polarity, Cell Signalling and Cancer “Equipe Labellisée Ligue Contre le Cancer”, Marseille, France

Correspondence should be addressed to F. Jouret; eb.ca.glu@teruoj.siocnarf

Received 30 March 2017; Accepted 21 May 2017; Published 11 July 2017

Academic Editor: Bruno Christ

Copyright © 2017 P. Rowart et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure. Panel A. Representative immunoblotting of phospho-AMP-activated protein kinase (pAMPK) and total AMPK (AMPKt) in low-Ca2+ conditions (S-MEM) and following Ca2+ switch using epithelial MDCK cells exposed to [10μM] and [50μM] compound C. Panel B. Representative immunoblotting of phospho-AMP-activated protein kinase (pAMPK) and total AMPK (AMPKt) at baseline (α-MEM), in low-Ca2+ conditions (S-MEM) and following Ca2+ switch using epithelial MDCK cells or mesenchymal stromal cells (MSC) or MDCK/MSC co-cultured cells. Each SDS Page was ran and transferred at the same time and incubated with the same primary and secondary antibodies for the same times. Panel C. Representative immunofluorescence of ZO-1 in MSC cultured alone and MDCK cultured alone. ZO-1 experiments were realized simultaneously using the same reagents for the same time of incubation.

  1. Supplementary material