Neuropilin 1 Mediates Keratinocyte Growth Factor Signaling in Adipose-Derived Stem Cells: Potential Involvement in Adipogenesis

Figure 6

FGFR2-IIIb and FGFR2-IIIc expression in ASCs. (a) FGFR2-IIIb and FGFR2-IIIc gene expression in ASCs were measured by absolute quantitation real-time PCR. MCF-7 cells were used as a positive control for FGFR2-IIIb and HFs as positive control for FGFR2-IIIc. (b) Western blot analysis of FGFR2-IIIb protein levels in ASCs, HFs, and MCF-7 cells. FGFR2-IIIb protein expression was evaluated by blotting with an anti-Bek antibody or with the homemade FGFR2-IIIb-specific SC-101 mAb. Western blot with anti-tubulin antibody was used as loading control. The images are representative of at least three independent experiments.

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