Research Article

Neuropilin 1 Mediates Keratinocyte Growth Factor Signaling in Adipose-Derived Stem Cells: Potential Involvement in Adipogenesis

Figure 7

Effect of FGFR2 silencing on KGF-mediated phosphorylation of ERK. (a) ASCs transfected with FGFR2-specific siRNA (siBek) or nonspecific control siRNA (siNC), treated or not with 20 ng/ml KGF for 5 min at 37°C, were lysed, and FGFR2 expression was analyzed by immunoblotting with anti-Bek antibodies. siBek induced a marked reduction in FGFR2 expression in both untreated and KGF-treated cells. Western blot with anti-tubulin antibodies was used as loading control. (b) The same lysates were analyzed by immunoblotting with anti-phospho-ERK antibody. Transfection with siBek did not affect ERK phosphorylation levels in KGF-treated cells. The levels of total ERK were assessed by Western blot with anti-ERK1/2 antibodies. The intensity of the bands was evaluated by densitometric analysis; the values from a representative experiment were normalized, expressed as fold increase with respect to the untreated siNC sample and reported as a graph. and .
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