IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model

Figure 1

Migratory capacity of AF-MSCs and IFNα-AF-MSCs toward cultivated HeLa cells. (a) Images of the AF-MSCs and IFNα-AF-MSCs that migrated toward HeLa cells, as determined by the Transwell migration assay. HeLa cells were precultured in lower wells of the Transwell chambers for 24 h. AF-MSCs, IFNα-AF-MSCs, and HSF were then plated in the upper well of the chambers. 24 h later, stem cells that had migrated to the membrane were fixed and stained with trypan blue. The cells were counted by microscopy. Bar, 100 μm. (b) Diagram of the migration capacity of AF-MSCs and IFNα-AF-MSCs. The cells of 4 high-power fields (×200) per membrane were counted manually. The data represent three independent experiments. Error bars, standard deviation, .

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