IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model

Figure 3

Induction of apoptosis of HeLa cells by IFNα-AF-MSCs in vitro. (a) Concentration of secreted IFNα in CM of IFNα-AF-MSCs of consecutive passage. IFN-α produced by IFNα-AF-MSCs and AF-MSCs was measured by ELISA in CM from P1-P5. The CM was collected 48 hours post transduction from three random wells. (b) Biological activity of the secreted IFNα on IFNα-AF-MSC proliferation. MSCs and IFNα-AF-MSCs from passages 5, 10, 15, and 20 were plated into 24-well plates at 5000 cells (500 μl) per well, to allow the cells to adhere to the plates. The cells from three random wells were counted for 8 days, and the mean values were used to assess cell proliferation. (c) Induction of apoptosis of HeLa cells for coculture with IFNα-AF-MSCs. AF-MSCs or IFNα-AF-MSCs were cocultured with HeLa cells in 12-well Transwell plates for 72 h. HeLa cells cultured alone were the negative control. After coculture, the apoptotic populations of HeLa cells were evaluated via FITC-labeled annexin V/PI staining flow cytometry. Apoptotic populations, including the early (annexin V+/PI−) and late (annexin V+/PI+) apoptotic cells, were assessed in the HeLa cells cocultured with AF-MSCs and IFNα-AF-MSCs. The data represent three independent experiments. Error bars, standard deviation , .

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