Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression

Figure 1

A multivariate approach to test the transfection efficiency of 6 commercially available cationic lipids and polymers using hBM-MSC #15. (a) Table of conditions for the 96-well multivariate transfection screening experiment with an untransfected control (UT) where no transfection reagent or DNA is added. (b) Representative 4X fluorescent overlay images of GFP+ cells (green) and Hoechst nuclei staining (blue) for the 6 commercially available cationic lipids and polymers assayed on hBM-MSCs. Images were taken after 24 hours at 2 different reagent/DNA (R/DNA) ratios per condition (1 and 2). Scale bars represent 1000 μm. (c) GFP fluorescence was quantified using a plate reader at 24 hours using 2 different R/DNA ratios for all 6 transfection conditions, along with their respective transfection agent control. (d) Total cell recovery quantification of all transfection conditions assayed on hBM-MSCs after 24 hours at 2 different R/DNA ratios and their respective transfection agent control. Cell recovery is expressed as a percentage of total cells measured in an experimental condition divided by total cells measured in the untransfected control. Results are from 4 independent experiments using 3 technical replicates with bars representing means ± S.E.M. Statistical significance was obtained using a one-tailed t-test for (c) and a one-way ANOVA with a Dunnet post hoc analysis for (d). and .

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