Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

Figure 2

H₂O₂ dose response in human adipose-derived mesenchymal stem cells. Percentage of cell viability and reactive oxygen species (ROS) in human adipose-derived mesenchymal stem cells (hADMSC) (a). DNA damage induced after 2 h of hydrogen peroxide, expressed as Olive tail moment (OTM) and corresponding to DNA breaks and alkali labile sites (DNA-SSB) and Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) in (b) (mean ± SD) (Supplementary Figure 1, comet assay images). To be precise, the corresponding incipient toxic doses of H₂O₂ were 0, 152.5, 305, 457.5, and 600 nmol of H₂O₂/mg cell protein. Data represent 3 independent duplicate experiments (). Nonparametric tests were performed using the Kruskal-Wallis test (one-way ANOVA on ranks), and all pairwise multiple comparison procedures were performed with Dunn’s test. DNA-SSB difference versus control (0 μM or 0 nmol/mg cell protein), . Fpg site difference versus control (0 μM), .

(a)

(b)