Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

Figure 5

H₂O₂-related DNA damage induction and DNA kinetic repair through adipocyte differentiation of hADMSCs. DNA breaks and alkali labile sites are represented in (a) (mean ± SD); DNA-Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) are shown in (b) (mean ± SD). Data represent 3 or 4 independent experiments with duplicates. Nonparametric tests were used for single cell gel electrophoresis analyses, the Kruskal-Wallis test employed one-way ANOVA on ranks, and all pairwise multiple comparison procedures used Dunn’s test. Differences versus controls correspond to the differentiation stage, and . The repair capacity of DNA breaks and alkali labile sites is shown in (c) (mean ± SD). The repair capacity of DNA-Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) is shown in (d) (mean ± SD). Student’s t-test versus hADMSCs, and . Images in Supplementary Figure 5.

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