Chronic Inflammation May Enhance Leiomyoma Development by the Involvement of Progenitor Cells

Figure 5

Indirect immunofluorescence analysis of α-SMA, collagen type 1, and fibronectin. A secondary FITC-conjugated antibody was used after incubation with the primary antibodies. Nuclei were counterstained with Hoechst 33342. Myometrium progenitor cells (MPCs) and leiomyoma progenitor cells (LPCs) showed a similarly strong positivity for α-SMA and fibronectin, whereas collagen type 1 expression was fainter. Differences between MPCs and LPCs were not significant (×200 original magnification).