Table 2: In vivo analysis of the integration efficiency of PGCs. To check the in vivo developmental potential of PG cells, we injected them back into 3-day-old recipient embryos. We investigated the integration rate of the injected PG cells in the 7-day-old embryo’s gonads. Using PC-derived PGCs, 50% percent of the gonads (a) contained the injected cells, while in the case of GFP PGC lines, we got 20% chimeric gonads (b). PC PGC line: Partridge colour Hungarian chicken embryo-derived primordial germ cell line. GFP PGC line: green fluorescent protein- (GFP-) expressing transgenic chicken embryo-derived primordial germ cell line.
(a)

Injection of PC PGCs into recipient embryosNumber%

Injected embryos32100.0
Chimera gonads1650

(b)

Injection of GFP PGCs into recipient embryosNumber%

Injected embryos69100.0
Chimera gonads1420