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Stem Cells International
Volume 2018, Article ID 1943980, 8 pages
https://doi.org/10.1155/2018/1943980
Research Article

Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment

1Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Ul. Waszyngtona 13, 15-269 Bialystok, Poland
2Department of Haematology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, Poland
3Department of Hematological Diagnostics, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, Poland
4Regional Centre for Transfusion Medicine, Ul. M. Skłodowskiej-Curie 23, 15-950 Bialystok, Poland
5Department of Gastroenterology, Pomeranian Medical University, Ul. Unii Lubelskiej 1, 71-252 Szczecin, Poland
6Department of Cardiology, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, Poland
7Department of Population Medicine and Prevention of Civilization Diseases, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, Poland
8Department of Allergology and Internal Medicine, Medical University of Bialystok, Ul. Waszyngtona 13, 15-276 Bialystok, Poland

Correspondence should be addressed to Andrzej Eljaszewicz; lp.ude.bmu@zciwezsajle.jezrdna and Marcin Moniuszko; lp.ude.bmu@okzsuinom.nicram

Received 6 March 2018; Accepted 3 June 2018; Published 21 June 2018

Academic Editor: Zengwu Shao

Copyright © 2018 Andrzej Eljaszewicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. Therefore, in this study we aimed to assess the effect of administration of G-CSF to ALL patients on mobilization of other than hematopoietic stem cell (HSCs) subsets, namely, very small embryonic-like stem cells (VSELs), endothelial progenitor cells (EPCs), and different monocyte subsets. Methods. We used multicolor flow cytometry to quantitate numbers of CD34+ cells, hematopoietic stem cells (HSCs), VSELs, EPCs, and different monocyte subsets in the peripheral blood of ALL patients and normal age-matched blood donors. Results. We showed that ALL patients following chemotherapy, when compared to healthy donors, presented with significantly lower numbers of CD34+ cells, HSCs, VSELs, and CD14+ monocytes, but not EPCs. Moreover, we found that G-CSF administration induced effective mobilization of all the abovementioned progenitor and stem cell subsets with high regenerative and proangiogenic potential. Conclusion. These findings contribute to better understanding the beneficial clinical effect of G-CSF administration in ALL patients following successful chemotherapy.