Research Article
3D Culture of Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) Could Improve Bone Regeneration in 3D-Printed Porous Ti6Al4V Scaffolds
Figure 2
Representation of passage 4 BMSCs on 2D culture plates (a) and in Matrigel 3D culture (b) (scale bar: 300 μm). Representative images of fluorescent staining for F-actin and nuclei (c and d). Blue: DAPI stain, red: rhodamine-phalloidin (scale bar 50 μm). The fluorescent quality of F-actin (e) and the nuclei number (f) was analyzed and compared using ImageJ 1.50i, and the mean of the IOD value was considered as the level of fluorescence intensity. , .
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