Location, Isolation, and Identification of Mesenchymal Stem Cells from Adult Human Sweat Glands

Figure 2

Histomorphology, immunocytochemical analysis, and ultrastructure of a detached solenoid bulb of ahSGs. (a) Phase contrast image of a detached ahSG solenoid bulb via phase contrast microscopy. (b) H&E staining of secretory and duct portions of the detached ahSG solenoid bulb. (c, d) Double immunofluorescence of the detached ahSG solenoid bulbs using antibodies against the following: CK15 ((c): red), CEA ((d): red), and α-SMA ((c, d): green) with cell nuclei stained by Hoechst 33342 (blue). White arrows indicated SMA positive cells. (e) The detached ahSG secretory portion by TEM. The ECM on the outside of the secretory portion of the detached ahSGs disappeared. (f) The detached ahSG secretory portion was observed by immunoelectron microscopy with anti-α-SMA as the primary antibody. Nu: nucleus; Myo: myoepithelial cells. White arrows indicated α-SMA positive immunogold labeling. (a) 40x. (b) Bar: 50 μm. (c, d) 400x. (e) Bar: 5 μm. (f) Bar: 2 μm.

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