Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages

<div>Intracellular protein activation at day 7 as a response to FAK, ROCK, and ERK inhibition. hASCs were cultured 7 days in BM, OM, and AM media containing 1% human serum supplemented with 15 <i>μ</i>M ROCK inhibitor, 2 <i>μ</i>M FAK inhibitor, or 30 <i>μ</i>M ERK inhibitor. (a) Representative WB results of immunoblotted p-FAK, FAK, <i>β</i>-actin, p-ERK(1/2), ERK 2, p-MLC2, and MLC2. (b) Semiquantified WB results representing the ratio of phosphorylated and basal form of FAK, ERK, and MLC2 proteins. BM: basic medium; OM: osteogenic medium; AM: adipogenic medium.</div>

Stem Cells International

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Figure 6

Figure 6: Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages 

Figure 6 | Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages 