Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages

Table 1

Culture media compositions.

Component	BM	OM	AM	Manufacturer
Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12)				Thermo Fisher Scientific, Waltham, MA, USA
GlutaMAX	1%	1%	1%
Insulin	—	—	100 nM
Human serum (HS)	5%	5%	5%	PAA Laboratories GmbH, Pasching, Austria
Penicillin/streptomycin	1%	1%	1%	Lonza, Basel, Switzerland
L-Ascorbic acid 2-phosphate	—	200 μM	—	Sigma-Aldrich, Saint Louis, MO, USA
β-Glycerophosphate	—	10 mM	—
Dexamethasone (DEX)	—	5 nM	1 μM
Pantothenate	—	—	17 μM
Biotin	—	—	33 μM
3-Isobutyl-1-methylxanthine (IBMX)	—	—	0.25 M