Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages

Table 3

Primary and secondary antibodies used in Western blot analysis.

Antibody type	Antibody	Host species	Dilution	Incubation
Primary	Anti-β-actin	Mouse	1 : 2000	RT, 2 h
Primary	Anti-FAK	Rabbit	1 : 1000	+4°C, overnight
Primary	Anti-p-FAK	Rabbit	1 : 1000	+4°C, overnight
Primary	Anti-ERK2	Rabbit	1 : 1000	RT, 2 h
Primary	Anti-p-ERK1/2	Rabbit	1 : 2000	+4°C, overnight
Primary	Anti-MLC2	Rabbit	1 : 800	+4°C, overnight
Primary	Anti-p-MLC2	Rabbit	1 : 800	+4°C, overnight
Secondary	Anti-rabbit IgG	Goat	1 : 2000	RT, 1 h
Secondary	Anti-mouse IgG	Goat	1 : 2000	RT, 1 h

Santa Cruz Biotechnology, Dallas, Texas, USA. Cell Signaling Technology, Danvers, Massachusetts, USA.