Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

Figure 1

SCAP isolation, culture, and identification. (a) The dental papillae were located at the apical part of the molars and showed pink in color. (b) The primary SCAP were isolated from the dental papilla tissues and displayed spindle-like morphological characters. (c) The 3rd passage SCAP were induced with osteogenic induction medium for 32 days and showed alizarin red-positive mineralized nodules. (d) The 3rd passage SCAP were induced with adipogenic induction medium for 16 days and showed oil red-positive lipid droplets. (e) The 3rd passage SCAP were analyzed by flow cytometry. Those SCAP presented positive expression of STRO-1, CD146, and CD24, but the expression of CD45 was negative.

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