B7-H1 Expression Is Required for Human Endometrial Regenerative Cells in the Prevention of Transplant Vasculopathy in Mice

Figure 4

B7-H1 is required for ERCs to increase the percentage of Tol-DCs in splenocytes. Splenocytes were collected from B6 recipients at posttransplant day 8, followed by double staining of anti-mouse CD11c antibody in combination with anti-mouse MHC class II or CD86 antibody for the FACS analysis. (a) Dot plots of (A) CD11c⁺MHC class II⁺DC and (B) CD11c⁺CD86⁺DC in each group. (b) Percentages of CD11c⁺MHC class II⁺DC and CD11c⁺CD86⁺DC in each group. (c) One-way MLR was used to measure the function of CD11c⁺ DCs (stimulator) from B6 recipients in each group. The proliferation index of CD3⁺ T cell (responders) was performed by the OD value. Values were presented as mean ± SEM; statistical analysis was done by one-way ANOVA followed by the LSD test, .

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