Inhibition of RAD51 by siRNA and Resveratrol Sensitizes Cancer Stem Cells Derived from HeLa Cell Cultures to Apoptosis

Figure 3

Inhibition of RAD51 decreases cell viability. (a) Western blot analysis showing RAD51 is overexpressed in HeLa SP cultures compared to the monolayer ones. (b) RAD51 expression is induced by Etoposide and efficiently inhibited by siRNA targeted to RAD51 (siRAD51). Western blot analysis shows that RAD51 expression is inhibited by increased amounts of siRAD51 (5, 10, 20, and 30 nM) in the presence of 5.8 μg/mL of VP16 for 48 h. (c) Inhibition of RAD51 expression in sphere culture sensitizes to VP16. Cell viability of spheres measured by MTT decreases with increasing concentrations of siRAD51 in the presence of VP16..

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