The promotive effect of N-cad upregulation on neurogenic differentiation of MenSCs. MenSCs were transfected with different plasmids, and the medium was replaced with fresh medium every 3 days. After cells were cultured for 14 days, immunofluorescence was performed. (a-d) and (i-l) EGFP-positive MenSCs in the pCAG-MCS-EGFP-treated group were negative for N-cad and S100 expressions. (e-h) and (m-p) EGFP-positive MenSCs in the human pCAG-N-cad-EGFP-treated group were positive for N-cad and S100 expressions.