Research Article

Metabolic Heterogeneity Evidenced by MRS among Patient-Derived Glioblastoma Multiforme Stem-Like Cells Accounts for Cell Clustering and Different Responses to Drugs

Figure 4

Lactate is differently extruded by different GSCs. (a) MR spectrum of culture medium for line #163, at day 4 after seeding; (b) behavior of Lac signal intensity as a function of time, starting at day 4 after seeding; (c) excreted lactate from the selected GSC lines as a function of the number of cells (N − N0, where N0 is the number of seeded cells); the goodness of the linear fit, R2, is in the range 0.96–0.99 for all the lines. (d) reports the rate of lactate extrusion (mM/cells) and mmoles of lactate for 1000 cells, respectively, for all four lines. Deconvolution of Lac signal regions is reported in Figure S2C.
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