Confirmation of differentiation of mouse mesenchymal stem cells (MSCs) into a neuronal phenotype as they stained positively for (b) β-III tubulin and NeuN. These differentiated neurons exhibited a phenotype resembling DA neurons as they positively stained for (d) tyrosine hydroxylase (TH) and β-III tubulin. Further, these differentiated DA neurons did not stain positively for (f) vimentin. Controls consisting of undifferentiated mouse MSCs did not stain positively for (a) NeuN and β-III tubulin and (c) TH and β-III tubulin, but they stained positively for (e) vimentin. Scale bar is 100 μm in all images.