IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway

Figure 4

Effect of IL-1β receptor antagonist, MMP-1 inhibitor, and MMP-1 siRNA on IL-1β-induced PAR1 expression. (a) Example of Western blot results of the active form of PAR1 (46 kDa) from the lysates of cells pretreated with TIMP1, TIMP2, and GM6001. (b) Example of Western blot results of PAR1 expression from the culture treated with IL-1β and IL-1RA. (c and d) Quantitative graphs of the Western blot results of the active form of PAR1 protein expression of (a) and (b), respectively. Data are shown as mean ± SD (, versus control, versus IL-1β). (e) Example of Western blot results of MMP-1 released in the medium after siRNA transfection with/without IL-1β stimulation. (f) Quantitative graphs of the Western blot results of MMP-1 released in the medium of (e). Data are shown as mean ± SD (, ). (g) MSCs transfected with MMP-1 siRNA attenuated PAR1 expression.

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