Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells

Figure 2

(a) Photomicrographs show different cell morphologies at different passages. At P0, spindle-shaped and polygonal cells were observed, and P1 cells demonstrated spindle-shaped fibroblastic morphology. At P3, homogeneous fibroblast-like cells were observed. Scale bars: 200 μm. (b) Phenotype of tendon-derived cells. RT-PCR was performed using total RNA extracted from P3 cells. The right column shows the results with total RNA from TDSCs. Total RNA extracted from turkey white blood cells was used as a control (left column). (c) Gene expression analysis of embryonic stem cell markers PouV, Nanog, and Sox2 at different cell passages. as compared to P3 cells.

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