Research Article
Quiescent Human Mesenchymal Stem Cells Are More Resistant to Heat Stress than Cycling Cells
Figure 4
DNA damage response (DDR) of eMSC heated in proliferating and quiescent stages. (a) Immunofluorescence assay of proliferating and quiescent cells with anti-γH2AX and anti-Ki-67 antibodies. Nuclei were contrasted with DAPI; scale bar = 25 μm. (b, c) Quantitation of γH2AX and anti-Ki-67 positive cells in proliferating (b) and quiescence (c) cultures after HS. At least 300 cells from different fields of view were counted. (d) qRT-PCR analysis of the ATR level in proliferating and quiescent cells exposed to HS. Mean ± SD of three independent experiments is presented. The difference vs unheated cells (t-test, ).
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