Research Article
Quiescent Human Mesenchymal Stem Cells Are More Resistant to Heat Stress than Cycling Cells
Figure 6
Oxidative defense in quiescent and proliferating cells after HS. (a) H2DCFDA-based FACS analysis of the ROS level in proliferating and quiescent cells. Analysis was performed 24, 48, and 72 h after treatment; all data were normalized to the control values. The difference vs unheated cells at the day of treatment. One-way ANOVA with post hoc Tukey HSD test, . (b, c) qRT-PCR analysis of the SOD1 and catalase gene level in proliferating and quiescent cells. The difference vs unheated cells (t-test, ). (d) RT-PCR analysis of the GPX, PRDX4, and TXN2 levels in proliferating cells. (e) RT-PCR analysis of the GPX, PRDX4, and TXN2 gene levels in quiescent cells. Data of three independent experiments are presented.
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