Stem Cells International / 2018 / Article / Fig 2

Research Article

A Loss of Function Screen of Epigenetic Modifiers and Splicing Factors during Early Stage of Cardiac Reprogramming

Figure 2

Knockdown of Sf3a1 or Sf3b1 inhibited the generation of iCMs. (a) Flow cytometry analysis (left) for αMHC-GFP+ and cTnT+ cells reprogrammed from ExCFs 10 days after infection of MGT with shNT, shSf3a1, or shSf3b1. The histograms (right) showed percentage and normalized fold change of αMHC-GFP+ and/or cTnT+ cells measured by flow cytometry. (b) Flow cytometry analysis (left) for αMHC-GFP+ and cTnT+ cells reprogrammed from fCFs 10 days after infection of MGT with shNT, shSf3a1, or shSf3b1. The histogram (right) showed percentage and normalized fold change of αMHC-GFP+ and/or cTnT+ cells measured by flow cytometry. (c) Representative ICC images for αMHC-GFP+ and αActinin+ cells on MGT-transduced ExCFs coinfected with shNT, shSf3a1, or shSf3b1. Scale bar, 100 μm. (d) ICC quantification of percentage and cell number of αMHC-GFP+ cells indicated in (c). (e) ICC quantification of percentage and cell number of cells expressing both αMHC-GFP+ and αActinin+ in (c) samples. (f) Total cell number of cells in (c) labeled by Hoechst 33342. , , and .
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