Stem Cells International / 2018 / Article / Fig 5

Research Article

A Loss of Function Screen of Epigenetic Modifiers and Splicing Factors during Early Stage of Cardiac Reprogramming

Figure 5

Knockdown of epigenetic factors changed the molecular features of reprogrammed cells. (a) Relative expression of cardiomyocyte-related sarcomere and ion channel genes or fibroblast marker genes in ExCFs infected with MGT and EV, shBcor, shZrsr2, or shStag2 at 10 days after infections. EV, empty vector, was used as a negative control. (b) Relative expression of sarcomere, ion channel, and fibroblast marker genes in MGT-infected ExCF at day 10 postinfection of EV, shSf3a1, or shSf3b1. EV, empty vector, was used as a negative control. , , and . (c) Relative expression of cardiomyocyte-related sarcomere and ion channel genes or fibroblast marker genes in MEFs infected with MGT and indicated shRNAs at 10 days after infections. EV, empty vector, was used as a negative control. , , , and .
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