Stem Cells International

Research Article

A Loss of Function Screen of Epigenetic Modifiers and Splicing Factors during Early Stage of Cardiac Reprogramming

Table 1

Categories and functional description of shRNA target genes.
shRNA targetCategoryDescription
Kdm5c/Jarid1c/SmcxEpigenetic modulatorHistone H3 lysine 4 demethylase
Kdm5d/Jarid1d/SmcyEpigenetic modulatorHistone H3 lysine 4 demethylase
Kmt2a/Mll1Epigenetic modulatorMember of SET1/MLL complexes
Kmt2d/Mll2Epigenetic modulatorMember of SET1/MLL complexes
Kmt2e/Mll5Epigenetic modulatorMember of SET1/MLL complexes
Phf23Epigenetic modulatorRedear of H3K4me3/2
CtcfEpigenetic modulatorChromatin insulator
Bcorl1Epigenetic modulatorComponent of BCOR complex (subtype of PRC1, PRC1.1)
BcorEpigenetic modulatorComponent of BCOR complex (subtype of PRC1, PRC1.1)
Rad21Epigenetic modulatorComponent of cohesin complex
Stag2/SA2Epigenetic modulatorComponent of cohesin complex
Phf12Epigenetic modulatorComponent of EMSY/KDM5A/SIN3B complex
Sin3bEpigenetic modulatorComponent of EMSY/KDM5A/SIN3B complex
TrrapEpigenetic modulatorComponent of TIP60 complex
Yy1Epigenetic modulatorComponent of INO80 complex
Ruvbl2/Tip48Epigenetic modulatorComponent of INO80 subfamily (INO80, TIP60, and SWR complexes)
Ruvbl1/Tip49Epigenetic modulatorComponent of INO80 subfamily (INO80, TIP60, and SWR complexes)
Smarca5Epigenetic modulatorComponent of ISWI complex (SWI/SNF)
Tet1Epigenetic modulatorDNA hydroxymethyltransferase
Tet3Epigenetic modulatorDNA hydroxymethyltransferase
Sf1Splicing factorSplicing factor for spliceosome assembly
Sf3a1/PRP21Splicing factorSplicing factor for spliceosome assembly (U2 snRNP)
Sf3b1Splicing factorSplicing factor for spliceosome assembly (U2 snRNP)
U2af1Splicing factorSplicing factor for spliceosome assembly
Zrsr2/URPSplicing factorSplicing factor for spliceosome assembly