A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications

Figure 5

Ability of DB-ASCs to uptake and release paclitaxel. The graph reports the inhibitory activity exerted on pancreatic carcinoma CFPAC-1 cells by (a) the conditioned medium (CM) of nontreated cells (CM CTRL) and (b) the CM of DB-ASCs loaded with PTX (CM/PTX). The activity is compared to the inhibition exerted by pure PTX. The activity is expressed as percentage of CFPAC-1 proliferation referred to that of untreated cells (100% proliferation). The regression and the correlation coefficient (R²) of the dose-response kinetics are also reported. The box (c) reports the IC₅₀ value referred to PTX (ng/ml), the paclitaxel equivalent concentration (PEC), and the single cell paclitaxel release (CPR). See details in Materials and Methods. Values are expressed as mean ± standard deviation (SD) of three independent experiments.

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