Adipose-Derived Stromal Vascular Fraction/Xenohybrid Bone Scaffold: An Alternative Source for Bone Regeneration

Figure 2

In vitro osteoblastic differentiation of ASCs and SVF. ASCs and SVF were cultured in the absence (ctrl) or in the presence of osteogenic medium (OM), for 60 days. In ctrl cultures, ALP expression is negative in ASCs (a), whereas it is positive in SVF (b). In the presence of OM, both ASCs and SVF show ALP-positive cells (c, d). For both ASCs and SVF, von Kossa staining is negative in the ctrl (e, f) and show mineral nodules with OM (g, h). Magnification 5x.