Use of Self-Assembling Peptides to Enhance Stem Cell Function for Therapeutic Angiogenesis

Figure 1

Combination of MSCs with RADA in in vitro cultures, demonstrating that their combination showed lower cell viability than MSCs alone. (a) Representative cell culture showing formation of colonies by MSCs and the combination of MSCs and RADA (10x magnification). (b) Quantification of colonies formed by MSCs and MSCs + RADA. The combination group showed significantly lower colony-forming units than MSCs alone. (c) Representative cell culture showing survival of MSCs and the combination of MSCs and RADA after 160 hours of live culture (10x magnification), showing an overall lower number of cells in the combination group compared to MSCs alone. (d) The lower cell growth in the combination group is shown in this quantification of cell growth for MSCs alone and MSCs + RADA, as measured by live cell screening analysis. (e) Quantification of cell viability by MTT assay also demonstrates that MSCs + RADA showed significantly lower cell viability than MSCs alone when compared to control. for control versus all other groups. .

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