Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells
SOX2 overexpression upregulates cell cycle and pluripotency gene expression in RMCMO. qPCR-based measurement of gene expression of five cell cycle regulatory genes (a) and the pluripotency genes, NANOG (b) and OCT3/4 (c), in RMCMO derived from SOX2- (black columns) and control-transfected (white columns) PBMCs. Data for the gene of interest (mean ± SEM, , duplicates) were normalized to the gene expression level of β-actin. Statistical analysis between SOX2-transfected and empty vector-transfected cells was performed by unpaired Student’s -test.
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