Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells
RMCMO generated from SOX2-transfected PBMCs are efficiently redifferentiated into insulin-producing cells. (a) Standard RMCMO or RMCMO generated from SOX2 transfection of PBMCs (insulin) were cultured in islet cell-conditioning medium for 2 weeks and subsequently subjected to analysis of β cell-specific gene expression. (b) Insulin-producing cells obtained after redifferentiation of SOX2-transfected RMCMO (new insulin-secreting cells) or standard RMCMO were cultured with increasing glucose concentrations to determine their ability to secrete insulin. Insulin secretion was quantified from 1 × 105 cells. (c) Glucose-stimulated insulin secretion in intact islets for comparison. Insulin secretion was quantified from 10 islets. (d) Insulin secretion response of insulin-producing cells obtained after redifferentiation of SOX2-transfected RMCMO from four different donors to glucose stimulation. The stimulatory index was calculated as glucose-stimulated secretion/basal secretion. Data are presented as mean ± SEM of . Statistical analysis between basal and stimulated secretions was performed by unpaired Student’s -test.
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