Injected Human Muscle Precursor Cells Overexpressing PGC-1α Enhance Functional Muscle Regeneration after Trauma

Figure 1

PGC-1α induces upregulation of the expression of contractile genes in regenerating muscle. Immunohistological assessment of newly built muscle fibers (green), stained with sarcomeric α-actinin antibody (Cy3, red) over time ((a) GFP and (b) PGC-1α). RTPCR analysis showed enhanced α-actinin, MyHC1, MyHC2, and Desmin gene expression when PGC-1α was overexpressed at (c) early (9 ± 1 d), (d) midterm (18 ± 3 d), and (e) late (31 ± 3 d) time points after the TA crush injury. TAcr: tibialis anterior crushed. and .

(a)

(b)

(c)

(d)

(e)