Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects

Figure 3

The chondrogenic differentiation potential of hUSCs in vitro. (a) Polysaccharides and proteoglycans were observed by toluidine blue staining after chondrogenic induction. (b) Immunofluorescence assay of chondrogenic-related markers (aggrecan and collagen II). (c) The mRNA expression of chondrogenesis-related genes (aggrecan, Sox9, and collagen II) was quantitated in hUSCs after 21 days of induction.

(a)

(b)

(c)