Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments
Table 2
Expression levels of integrins in hMSCs cultured for 28 days in 2D standard and 3D ECM-based structure conditions.
Gene
Average FPKM
Significance
2D/D28
3D/BSA
3D/FN
3D/Col1
3D/LN
ITGA1
1.28
5.34
2.93
5.12
7.34
ITGA2
2.18
9.54
4.37
4.18
11.33
ITGA3
1.40
0.55
0.31
0.72
0.39
ITGA4
0.68
15.22
3.15
2.55
0.63
a
ITGA5
62.99
36.35
24.16
25.63
55.23
ITGAE
19.68
16.17
17.53
11.81
16.99
ITGAV
3.89
17.92
13.74
33.58
18.13
c, g
ITGA6
0.60
2.49
7.93
3.76
0.96
ITGA7
0.15
2.82
0.23
0.18
0.00
ITGA8
1.81
0.00
0.38
1.74
1.79
ITGA10
3.98
20.99
19.55
27.72
50.63
d
ITGA11
33.87
25.68
18.59
46.22
50.06
ITGB1
280.32
547.67
456.69
572.81
551.84
ITGB2
0.16
0.00
0.59
6.30
0.00
ITGB4
0.00
0.00
0.50
2.84
0.00
ITGB5
37.11
27.88
25.67
29.49
22.85
ITGB8
0.68
12.44
4.52
11.86
6.27
Bold: reduction in expression, compared to 2D standard. Italic: increase in expression, compared to 2D standard. Underlined: FPKM < 1. a: 2D versus BSA; b: 2D versus 3D/FN; c: 2D versus 3D/Col1; d: 2D versus 3D/LN; e: BSA versus 3D/I; f: BSA versus 3D/LN; g: 3D/FN versus 3D/Col1; h: 3D/FN versus 3D/LN.
